Ultrastructural studies of frozen-thawed 8-cell mouse embryos
نویسندگان
چکیده
منابع مشابه
In vitro development and gene expression of frozen-thawed 8-cell stage mouse embryos following slow freezing or vitrification
OBJECTIVE This study was performed to compare the efficiency of slow freezing and vitrification based on survival, development to blastocysts, and cell numbers of blastocysts. Changes in embryonic gene expression in fresh and frozen-thawed embryos were also examined. METHODS Eight-cell stage embryos were collected from superovulated female BDF1 mice. The collected embryos were randomly divide...
متن کاملCharacteristics of the cell membrane fluidity, actin fibers, and mitochondrial dysfunctions of frozen-thawed two-cell mouse embryos.
Physical and chemical alterations caused by the freezing and thawing and their effects on survivals/developments in vitro were investigated. Of a total of 452 two-cell mouse embryos, the overall survival rate of the frozen-thawed embryos was 76.1% (344/452). The blastocyst formation of the frozen-thawed embryos was 32.6% (44/136) compared to 74.5% (117/157) in the fresh embryos (P<0.05). The to...
متن کاملHuman pregnancy after transfer of intact frozen-thawed embryos.
Since the birth of the first baby as a result of in vitro fertilisation (IVF) in 1978, many clinics around the world have achieved pregnancies and births for their patients by using IVF and gamete intrafallopian transfer procedures. With the storage of excess embryos, multiple laparoscopies can be avoided; this has favoured the development of better cryopreservation techniques. In our clinic 8-...
متن کاملMouse embryos generated from frozen-thawed oocytes can successfully survive a second cryopreservation.
BACKGROUND To determine whether mouse embryos generated from frozen-thawed oocytes can successfully survive a second cryopreservation. METHODS Immature C57BL6*BALB/c female mice underwent superovulation and the collected oocytes were divided into three groups. Group A oocytes (n = 107) underwent IVF. Group B oocytes (n = 167) underwent IVF and embryos generated were then cryopreserved. Group ...
متن کاملO-23: Transfer of Blastocysts Derived from Frozen Thawed Cleavage Stage Embryos Improved Ongoing Pregnancy
Background: The aim of our study was to compare the resulting from transfer of embryos that cryopereserved in cleavage stage after thawing and transfer of embryos after thawing and culture in sequentional media until blastocyst formation. Materials and Methods: In this prospective clinical study, we have evaluated 128 cycles in women undergone in vitro fertilization (IVF) or intracytoplasmic sp...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1976
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0480137